|
220
The Major Allergen from Birch Tree Pollen, Bet v 1, Binds and Permeabilizes Membranes†
The Major Allergen from Birch Tree Pollen, Bet v 1, Binds and Permeabilizes Membranes†
|
|
Jesper E. Mogensen,‡§ Mercedes Ferreras,‖ Reinhard Wimmer,‡ Steen V. Petersen, Jan J. Enghild, and D
2007
|
|
The 159 residue Bet v 1 is the major allergen from birch tree pollen. Its natural function is unknown although it is capable of binding several types of physiologically relevant ligands in a centrally placed cavity in the protein structure. Here we use circular dichroism and fluorescence spectroscopy to show that Bet v 1 binds to DOPC and DOPG phospholipid vesicles in a pH-dependent manner. Binding is facilitated by low pH, negatively charged phospholipids, and high vesicle curvature, indicating...
|
| 查看全文
|
|
|