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The tritiated chiral alkanes (S)-[1-2H1,1-3H]ethane, (R)-[1-2H1,1-3H]ethane, (S)-[1-2H1,1-3H]butane, (R)-[1-2H1,1-3H]butane, (S)-[2-3H]butane, (R)-[2-3H]butane, and racemic [2-3H]butane were oxidized by soluble methane monooxygenase (sMMO) from Methylococcus capsulatus (Bath), and the absolute stereochemistry of the resulting product alcohols was determined in order to probe the mechanism of substrate hydroxylation. When purified hydroxylase, coupling protein, and reductase components were used,...
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